Manufacturer,provider and supplier of
Recombinant Human Granulocyte Colony Stimulating Factor,G-CSF,GCSF,rHuG-CSF

Source   A DNA sequence encoding the mature human G-CSF protein sequence was expressed in E. coli.
Molecular Mass The methionyl form of the E. coli expressed mature G-CSF contains 175 amino acid residues and has a predicted molecular mass of approximately 18.8kD.
Purity > 97%, as determined by SDS-PAGE and HPLC method.
Endotoxin Level  <10EU/0.3mg of the cytokine as determined by the LAL method.
Activity Measured in a cell proliferation assay using NFS-60 cell line.The ED50 for this effect is typically 0.02 - 0.06ng/mL.
Formulation Lyophilized from a 0.2µm filtered solution of 10mM acetic acid containing 50µg of human serum albumin per 1µg of cytokine.
Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 µg/mL of the cytokine.
Storage  Lyophilized samples are stable for greater than six months from date of receipt at -20 to -70ºC .
Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8ºC for up to one month without detectable loss of activity.
DO NOT FREEZE AFTER RECONSTITUTION! Loss of activity,estimated at 60 - 75%,has been observed upon thawing.
Size and prices 100ug US$200,1.0mg US$1,100

Human granulocyte colony-stimulating factor (G-CSF) is produced by recombinant DNA technology.In comparison with natural products, its bioactivity is similar in vivo & in vitro. rHuG-CSF is one of the main cytokines modulating the granulocytic hematopoiesis in bone marrow. It acts on the hematologic progenitor cells of granulocyte selectively,promoting its proliferation and differentiation.It enhances the function and counts of granulocyte in peripheral blood as well.In vitro,G-CSF stimulates growth, differentiation and functions of cells from the
neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.